Fmoc-L-Lys[Ste(OtBu)-γ-Glu-(OtBu)-AEEA-AEEA]-OH

Fmoc-L-Lys[Ste(OtBu)-γ-Glu(OtBu)-AEEA-AEEA]-OH

Tšebeliso ea Lipatlisiso:
Motsoako ona ke motsoako oa lysine o fetotsoeng o sebelisoang ho peptide synthesis, haholo-holo bakeng sa ho aha li-conjugate tsa peptide tse lebisitsoeng kapa tse sebetsang ka bongata. Sehlopha sa Fmoc se lumella ho qaptjoa ha mohato ka mohato ka Fmoc solid-phase peptide synthesis (SPPS). Ketane ea mahlakoreng e fetotsoe ka motsoako oa stearic acid (Ste), γ-glutamic acid (γ-Glu), le lihokelo tse peli tsa AEEA (aminoethoxyethoxyacetate), tse fanang ka hydrophobicity, thepa ea tefiso, le sebaka se feto-fetohang. Hangata e ithutoa ka karolo ea eona litsamaisong tsa phano ea lithethefatsi, ho kenyeletsoa li-antibody-drug conjugates (ADCs) le li-peptide tse kenang liseleng.

Mosebetsi:
Fmoc-L-Lys[Ste(OtBu)-γ-Glu(OtBu)-AEEA-AEEA]-OH e sebetsa e le moaho bakeng sa ho hlahisa li-peptide tse nang le ketane e telele kapa li-complex-linker complexes. Asiti ea stearic e ntlafatsa kamano ea lera, γ-Glu e ntlafatsa botsitso le ho hanyetsa ha enzymatic, 'me lihokelo tsa AEEA li fana ka solubility le ho feto-fetoha ha sebopeho. Ka kopanelo, likarolo tsena li etsa hore motsoako o be oa bohlokoa ho rala li-peptide bakeng sa bioavailability e ntlafalitsoeng, tokollo e laoloang, le lits'ebetso tse reriloeng tsa phano.