Fmoc-L-Lys[Eic(OtBu)-γ-Glu(OtBu)-AEEA]-OH

Fmoc-L-Lys[Eic(OtBu)-γ-Glu(OtBu)-AEEA]-OH

Tšebeliso ea Lipatlisiso:
Motsoako ona ke motsoako o sirelelitsoeng, o sebetsang oa lysine o sebelisoang ho peptide synthesis le nts'etsopele ea conjugate ea lithethefatsi. E na le sehlopha sa Fmoc bakeng sa tšireletso ea N-terminal, le phetoho ea ketane ea mahlakoreng ka Eic (OtBu) (eicosanoic acid derivative), γ-glutamic acid (γ-Glu), le AEEA (aminoethoxyethoxyacetate). Likarolo tsena li etselitsoe ho ithuta litlamorao tsa lipidation, chemistry ea spacer, le tokollo ea lithethefatsi e laoloang. E batlisisoa haholo molemong oa maano a lihlahisoa, lihokelo tsa ADC, le li-peptide tse sebetsanang le membrane.

Mosebetsi:
Fmoc-L-Lys[Eic(OtBu)-γ-Glu(OtBu)-AEEA]-OH e sebetsa e le mohaho oa mohaho bakeng sa ho kopanya li-peptide tse fetotsoeng ka lipid kapa mekhoa ea ho fana ka lithethefatsi. Eic moiety e ntlafatsa lipophilicity le membrane tlamahano; γ-Glu e eketsa botsitso ba enzymatic le bokhoni ba ho lebisa tlhokomelo; mme AEEA e sebetsa e le sehokelo sa hydrophilic se tenyetsehang. Ka kopanelo, li thusa ho aha li-peptide tse nang le ts'ebeliso e ntlafalitsoeng ea cellular, bophelo bo atolositsoeng ba halofo ea bophelo, le thepa e ikhethileng ea sebaka.